理学院学术报告—Carbon dots doped with nitrogen as an ultrasensitive fluorescent probe for thrombin activity monitoring and inhibitor screening

报告题目：Carbon dots doped with nitrogen as an ultrasensitive fluorescent probe for thrombin activity monitoring and inhibitor screening

报  告 人：赵艳

报告时间：2023年5月17日下午14:00

报告地点：化学实验中心417

主办单位：理学院

报告人简介：

赵艳，女，博士，教授，硕士生导师。主要研究方向为新型纳米材料的制备和性质研究及其在生物活性成分、污染物分析方面的应用。先后在Food Chem.、Talanta、Microchem. J.等国际期刊发表SCI论文10余篇。

报告摘要：

A simple and sensitive fluorometric assay based on nitrogen-doped carbon dots (N-CDs) was developed for the determination of thrombin (TB) activity in human serum samples and living cells. The novel N-CDs were prepared by a facile one-pot hydrothermal method using 1,2-ethylenediamine and levodopa as precursors. Such N-CDs exhibited green fluorescence with excitation/emission peaks at 390/520 nm and a high fluorescence quantum yield of approximately 39.2%. H-D-Phenylalanyl-L-pipecolyl-Larginine-p-nitroaniline-dihydrochloride (S-2238) was hydrolyzed by TB to produce p-nitroaniline which was capable of quenching the fluorescence of N-CDs due to an inner filter effect. This assay was used to detect TB activity with a low detection limit of 11.3 fM. The proposed sensing method was then expanded to the TB inhibitor screening and exhibited excellent applicability. As a typical TB inhibitor, argatroban was determined in a concentration as low as 1.43 nM. The method has also been successfully employed for the determination of TB activity in living Hela cells. This work showed significant potential for TB activity assay in clinical and biomedicine applications.